9wishes Pine Perfect Ampule Serum

Your skin makes glycerin on its own (mostly from sebaceous oil breakdown) and shuttles it to your outermost layer of skin, or your epidermis, via aquaporin-3.

Aquaporin-3 is a transporter that is essential for normal skin hydration, elasticity, and repair. Interestingly, mice lacking in AQP3 have dry and less elastic skin that can be fully corrected with glycerin.

This ingredient is non-irritating, plays well with almost every ingredient, and works across all skin types. Typical use is anywhere between 3-10% but can go up to 79% in some leave-on products.

Just know very high concentrations (>40%) can feel tacky in low humidity.

Glycerin is the name for this ingredient in American English. British English uses Glycerol/Glycerine.

Though this ingredient works well with most skin types, some people with sensitive skin may experience a reaction such as allergic rashes, closed comedones, or itchiness.

Dipropylene glycol is technically an alcohol, but it belongs to the glycol family (often considered part of the ‘good’ alcohols). This means it is hydrating and gentle on skin unlike drying solvent alcohols like denatured alcohol.

As a masking agent, Dipropylene Glycol can be used to cover the smell of other ingredients. However, it does not have a scent.

Studies show Dipropylene Glycol is considered safe to use in skincare.

Due to its antioxidant properties, trehalose may help with signs of aging. Antioxidants help fight free-radical molecules, unstable molecules that may damage your skin.

In medicine, trehalose and hyaluronic acid are used to help treat dry eyes.

Some animals, plants, and bacteria create trehalose as a source of energy to survive freeze or lack of water.

Some studies suggest betaine may support making skin tone more even.

Fun fact: Betaine naturally exists in the skin and the body. In cosmetic products, it can be either plant-derived (most commonly from sugar beets) or synthetically produced for consistency and stability.

Betaine is also known as trimethylglycine.

Interestingly, the size of the molecule affects its behavior:

Some clinical evidence links low molecular weight versions to improved wrinkle depth, elasticity, anti-inflammatory effects, and barrier repair.

Many serums use a blend of both weights so you can get surface hydration plus longer-lasting and deeper effects.

You'll typically see concentrations between 0.1-2% for this ingredient.

Studies show green tea extract and its catechins (like epigallocatechin gallate (EGCG)) help your skin cells product energy more efficiently and reducing the number of free-radicals that can damage your skin from the inside.

In lab-grown skin models, this translated to younger, healthier, and stronger skin.

There's also good sun protection data; researchers saw less DNA damage and redness on human skin when green tea was applied before UVB exposure. And the more they applied, the better the protection.

Needless to say, this ingredient shouldn't replace your sunscreen. But it is a great supportive ingredient that you can already find in many sunscreens and antioxidant serums.

A 2009 study found a 2% green tea lotion was effective for mild-to-moderate acne thanks to its anti-inflammatory and mild antimicrobial activity.

The quality of the extract matters a lot here:

Good extracts contain 50-90% catechins while lower quality ones are mostly there for marketing. We recommend reaching out to the brand if you have questions about the quality or source of their ingredients.

Human Repeated Insult Patch Testing showed no irritation or sensitization at use concentrations (0.86% in leave-on products and up to 30% as leaf water).

Studies show Laminaria Japonica has antioxidant compounds. Antioxidants may help with anti-aging. Kelp also contains magnesium and zinc, making it great for calming inflammation and redness.

A study from 2021 found this ingredient to inhibit melanin production. An animal study found plum blossom to help increase collagen production.

Broccoli is also rich in fatty acids such as oleic and linoleic acids.

Brassica Oleracea Italica Extract can be extracted from any part of the broccoli plant.

Besides fatty acids, avocados also have B vitamins, vitamin K, vitamin C, vitamin E, and potassium.

They contain shikimate dehydrigenase, an enzyme shown to help reduce inflammation and soothe the skin.

The amino acids found in cucumbers help nourish our skin's natural acid mantle (it's an important part of our skin barrier). This slightly acidic film acts as a barrier to protect us from bacteria, viruses, and other contaminants.

Unless you have an allergy to cucumbers, this is generally a non-irritating ingredient.

Fun fact: Cucumis Sativus is native to South Asia and can now be found on every continent.

Because it is not a free-fatty acid or alcohol, this ingredient is fungal acne safe (there's nothing for Malassezia to feed on).

It can also form a thin protective film on skin. One study found that a formula using this polymer helped slow down how quickly other ingredients (like DEET) were absorbed through skin.

A 2024 study of over 1,300 patients confirmed that sensitization to this ingredient is rare. It is also non-mutagenic and has a clean track record.

Tromethamine helps balance the pH level of a product. Normal pH level of skin is slightly acidic (~4.75-5.5). The acidity of our skin is maintained by our glands and skin biome. Being slightly acidic allows our skin to create an "acid mantle". This acid mantle is a thin barrier that protects our skin from bacteria and contaminants.

Oral Tromethanmine is an anti-inflammatory drug but plays the role of masking, adding fragrance, and/or balancing pH in skincare.

1,3-Propanediol, 2-amino-2-(hydroxymethyl)-

Snow mushroom is rich in polysaccharides, giving it moisturizing benefits. As a humectant, snow mushroom helps draw moisture from the air to the skin. Polysaccharides are able to mimic our skin's natural carbohydrates to help the skin retain water.

Snow mushroom has been shown to help with anti-aging due to its antioxidants. It increases the presence of superoxide dismutase in our brain and liver. Superoxide is a by-product of oxygen metabolism. It left unregulated, superoxide causes cell damage. Thus, superoxide dismutase acts as an antioxidant to protect our living cells.

Another study from 2016 showed snow mushroom extract blocks the skin from creating melanin when exposed to UVB. Thus, it may help protect against the signs of aging from sun exposure. However, more studies are needed to confirm this. Another study from 2021 found snow mushroom to protect against UVA damage as well.

This white mushroom is native to Asian countries such as China, Korea, Japan, and Thailand. It is a common ingredient in traditional Chinese cuisine and medicine.

Licorice Root also contains a flavonoid. Flavonoids are a natural substance from in plants. Flavonoids also have antioxidant properties.

Another component, glycyrrhizin, has been found to have anti-inflammatory and antimicrobial benefits. This may make licorice root extract effective at treating acne. However, more research is needed to support this.

Liquiritin is one of the flavone compounds found in licorice. It has been found to help lighten skin by preventing tyrosinase from reacting with tyrosine. When the two react, protein is converted to melanin. Melanin is the substance in your body that gives your features pigmentation.

Research has also shown that myrrh can help calm itch and irritation by dialing down your skin's inflammatory response at the cellular level.

In lab studies, myrrh extract helped close wounds by up to 98.4%. This suggests it has real potential for supporting skin repair and recovery.

Fun fact: This ingredient has been used for centuries to treat wounds, skin infections, and inflammatory conditions.

This plant contains the compounds saponins, glycosides, flavanoids, protein, triterpenoids.

The common wugwort has been used in traditional Indian, Chinese, and European medicines to treat gastrointestinal diseases.

Vaccinium Myrtillus Fruit Extract also helps reduce irritation.

Industry-reported use ranges from 8% in rinse-off products and 2% in leave-on formulations.

On top of that, this ingredient can counteract the effects of hard water by binding to the minerals in it.

One thing worth knowing is that Disodium EDTA has been shown to be a mild penetration enhancer. It can help other ingredients absorb into skin more effectively which can be a double-edged sword (great for actives, but can also make the active too strong if you have sensitive skin).

Clinical patch testing showed no significant skin irritation at typical use concentrations and minimal dermal absorption.

You'll most likely see this ingredient near the end of an ingredient list. It's typically found in concentrations less than 1%.

This property also makes Pentylene Glycol a great texture enhancer. It can also help thicken or stabilize a product.

Pentylene Glycol also acts as a mild preservative and helps to keep a product microbe-free.

Some people may experience mild eye and skin irritation from Pentylene Glycol. We always recommend speaking with a professional about using this ingredient in your routine.

Pentylene Glycol has a low molecular weight and is part of the 1,2-glycol family.