Biotinoyl Tripeptide-1

Biotinoyl Tripeptide-1 is a lab-made molecule that combines two parts: biotin (vitamin B7) and Tripeptide-1. Together, they form a peptide that’s often used in hair and eyelash products.

In-vitro studies show this ingredient helps hair bulb cells grow faster and product more structual proteins. This helps keep hair securely anchored in the follicle.

A small human study using a mascara with 2% Biotinoyl Tripeptide-1 reported lashes that were about 17% longer and 19% thicker after one month.

One study from 2025 compared topical spray vs. a combined tropical/oral treatment. The combination group saw greater increases in total hair count.

While these early results are encouraging, most of the data comes from in-vitro experiments (in test tubes) or small, short-term trials.

Biotinoyl Tripeptide-1 looks promising for supporting hair strength and growth but there’s not yet strong clinical evidence to confirm how well it works.